NMImETIC Alizarin Red
10CC= $7
NMImETIC Alizarin Red stain is a premium-grade, ready-to-use solution optimized for the qualitative and quantitative assessment of calcium deposition in mineralized tissues and cell cultures. This product is specifically designed for researchers studying the osteogenic differentiation of mesenchymal stem cells (MSCs) into osteoblast-like cells. NMImETIC Alizarin Red S binds specifically to calcium ions within the mineralized extracellular matrix, enabling precise visualization and quantification of calcium deposition. The intensity of staining correlates directly with the extent of mineralization. Our formulation ensures superior staining performance and reduced background noise compared to standard Alizarin Red S solutions. Elevate your research with our NMImETIC Alizarin Red—order yours today!
Intended Use
NMImETIC Alizarin Red S Stain is intended for in vitro use in research settings to assess the osteogenic differentiation potential of MSCs. It is suitable for both qualitative (visual assessment) and quantitative (colorimetric measurement) analysis of calcium deposition and is not for diagnostic or therapeutic procedures. For safety precautions please see appropriate SDS.
Place your order today and take the first step toward advancing your studies in bone biology.
Notes
- If the Alizarin Red stain is not fully dissolved, transfer the entire contents of the well (precipitate and solution) to a 1.5 mL microcentrifuge tube. Seal the tube with Parafilm, incubate at 85°C for 10 minutes, then cool on ice for 5 minutes. Transfer 100 µL to a 96-well plate for absorbance reading.
- If a 405 nm absorbance reader is unavailable, neutralize the acidic solution (400 µL acetic acid) by adding 150 µL of 10% ammonium hydroxide or 1% sodium hydroxide until the solution turns from yellow to purple (pH neutral). Measure absorbance between 540-630 nm, preferably at 570 nm
- For storage exceeding six months, verify the pH indicator and adjust to 4.1-4.3 using 1% NaOH as needed.
Order Now!
Unlock nature’s potential for healing and enhance your research outcomes with our NMImETIC Alizarin Red. Visit our website to place your order today and take the first step toward advancing your studies in bone biology!
Volume
10 ml, 25 ml, and 50 ml
Price
10ml=480,000 Tomans
10ml= $7
Qualitative Assay Instructions
Materials:
- NMImETIC Alizarin Red S Stain (this product)
- Cell culture plates (e.g., 6-well, 24-well) containing MSC-derived osteoblast-like cells
- Phosphate-buffered saline (PBS)
- 70% ethanol, 10% formaldehyde or 4% paraformaldehyde (fixative of choice)
- Distilled water
- Microscope or imaging system
Procedure:
Remove Culture Media: Carefully aspirate the culture media from the cell culture plates.
Wash Cells: Gently wash the cells twice with PBS.
Fix Cells: Add fixative to the wells and incubate for at least 30 minutes at room temperature to fix the cells.
Remove Fixative: Aspirate the fixative and wash the cells twice with distilled water.
Stain Cells: Add NMImETIC Alizarin Red solution to completely cover the cells. Incubate for 5-10 minutes at room temperature. Optimal incubation time may need to be determined experimentally.
Remove Stain: Aspirate the NMImETIC Alizarin Red. Wash the cells gently with distilled water several times until the background is clear.
Visualize: Observe the stained calcium deposits under a microscope or imaging system. Red staining indicates the presence of calcium deposits. Image acquisition should be performed consistently to allow for comparison between samples.
Quantitative Assay Instructions
Materials:
- NMImETIC Alizarin Red
- Cell culture plates (e.g., 96-well) containing MSC-derived osteoblast-like cells
- Phosphate-buffered saline (PBS)
- 10% acetic acid
- Spectrophotometer
Procedure for 96- well plate
Remove Culture Media: Carefully aspirate the culture media from the cell culture plates.
Wash Cells: Gently wash the cells twice with PBS.
Fix Cells: Add fixative to the wells and incubate for at least 30 minutes at room temperature to fix the cells.
Remove Fixative: Aspirate the fixative and wash the cells twice with distilled water.
Stain Cells: Add 50 µl NMImETIC Alizarin Red solution to completely cover the cells. Incubate for 5-10 minutes at room temperature. Optimal incubation time may need to be determined experimentally.
Remove Stain: Aspirate the NMImETIC Alizarin Red. Wash the cells gently with distilled water 2 times until the background is clear.
Elution: Add 400µl of 10% acetic acid to the wells to elute the bound Alizarin Red S.
Incubate for 30 minutes at 37 °C.
Read Absorbance: Transfer the eluted solution to a new plate and measure the absorbance at 405 nm using a spectrophotometer.
Calculate: Determine the amount of calcium deposition based on a standard curve generated using known concentrations of calcium. Include appropriate controls (e.g., positive and negative controls) in your experiment.
Data Sheet NMImETIC Alizarin Red
SDS NMImETIC Alizarin Red
Join the Revolution in Bone Regeneration
Experience the transformative power of our NMImETIC Osteogenic Differentiation Medium and elevate your research to new heights. Join countless satisfied researchers who have successfully utilized our medium to achieve remarkable results in bone regeneration studies.
Alizarin Red Stain
5ml
Sirus Red Stain
Cell Culture Media
Cell Supernatant
5 ml
Papers with Osteogenic Differentiation Medium
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Core and biological motif of self-assembling peptide nanofiber induce a stronger electrostatic interaction than BMP2 with BMP2 receptor 1A
- A Porous Hydroxyapatite/Gelatin Nanocomposite Scaffold for Bone Tissue Repair: In Vitro and In Vivo Evaluation
-
Using osteogenic medium in the in vitro evaluation of bone biomaterials: Artefacts due to a synergistic effect
-
Osteogenic potential of Frondoside A in human periodontal ligament cells: an RNA-Seq analysis
-
Metformin enhances osteogenic differentiation of BMSC by modulating macrophage M2 polarization
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Upregulation of NOR-1 in calcified human vascular tissues: impact on osteogenic differentiation and calcification
News
Production of Three Bone-Forming Research Products at Iran University of Medical Sciences
In response to the needs of researchers in the country, three bone-forming research products have been developed at Iran University of Medical Sciences. According to the Public Relations Office of the Research and Technology Department, Dr. Shima Tavakkol, an assistant professor at the university’s Cellular and Molecular Research Center and CEO of Tavakkol BioMimetic Technologies, has produced sterile spherical hydroxyapatite nanoparticles compliant with ISO 13175-3 and ISO 13485 under the brand name NMImETIC nHA. Additionally, differentiation culture media for bone (NMImETIC Osteogenic Medium) and fat (NMImETIC Adipogenic Medium) have been created to alleviate the need for importing these products.
https://vcr.iums.ac.ir/Z5aEo
Production of Knowledge-Based Products: Spherical Hydroxyapatite Nanoparticles and Differentiation Culture Media by Iranian University Scientists
Researchers at Iran University of Medical Sciences, led by Assistant Professor Dr. Shima Tavakkol at the Cellular and Molecular Research Center, have developed sterile spherical hydroxyapatite nanoparticles for research applications. This initiative aims to meet the needs of Iranian researchers and reduce dependence on imported products.
According to the Public Relations Office of the Research and Technology Department, Dr. Tavakkol, who is also the CEO of the knowledge-based company Tavakkol Biotech, has produced these nanoparticles in compliance with ISO 13175-3 and ISO 13485 standards under the brand name NMImETIC nHA. Additionally, she has developed differentiation culture media for osteogenic and adipogenic applications, branded as NMImETIC Osteogenic Medium and NMImETIC Adipogenic Medium, respectively, to further support research needs in Iran.
https://news.iums.ac.ir/ZbaEo
Shima Tokal, CEO of a knowledge-based company, stated in an interview with Ana News Agency’s reporter: “We have had 14 years of research and development for 4 products, which have received pre-clinical and production line approvals from the Food and Drug Administration. These products are in the field of bone formation, and each one is unique in its own way”. This associate professor at Iran University of Medical Sciences explained: “There are no injectable bone-forming scaffolds in the world. If a bone fracture occurs, the surgeon is forced to open the area to allow the bone to heal. However, our company’s self-assembling nanofiber peptide product is liquid, and with the entry of this product into the market, injection into the fractured area will be possible without open surgery.
https://ana.ir/003xAe
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